Typing of Dengue Viruses in Clinical Specimens and Mosquitoes by Single-Tube Multiplex Reverse Transcriptase PCR

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Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR.

In recent years, dengue viruses (serotypes 1 to 4) have spread throughout tropical regions worldwide. In many places, multiple dengue virus serotypes are circulating concurrently, which may increase the risk for the more severe form of the disease, dengue hemorrhagic fever. For the control and prevention of dengue fever, it is important to rapidly detect and type the virus in clinical samples a...

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Typing of dengue viruses in clinical specimens from central Thailand.

Typing of dengue viruses was done for clinical specimens from a total of 136 patients (children under 15-years-old) suspected of having dengue virus infections and who had been admitted to Pathum Thani Provincial Hospital during the period May 1999 to April 2000. Altogether, 44 strains were isolated (isolation rate: 32.4%), consisting of 18 DEN-1, 18 DEN-2, 7 DEN-3 and 1 DEN-4. The isolation ra...

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Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.

We report on the development and application of a rapid assay for detecting and typing dengue viruses. Oligonucleotide consensus primers were designed to anneal to any of the four dengue virus types and amplify a 511-bp product in a reverse transcriptase-polymerase chain reaction (PCR). First, we produced a cDNA copy of a portion of the viral genome in a reverse transcriptase reaction in the pr...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1998

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.36.9.2634-2639.1998